was added to the mixture and maintained for
5 min. Then, 0.15mL of 10% aluminum chloride was
added. After 6 min, 0.5mL of 1M sodium hydroxide was
finally added. The mixture was diluted with 0.275mL of
distilled water. The absorbance of the mixture at 510 nm
was measured immediately in comparison to a standard
curve prepared by quercetin. The flavonoid contents were
expressed as mg quecetin equivalent (QE)/g dry basis.