The CHCl3 extract (2.4 g) was subje

The CHCl3 extract (2.4 g) was subjected to silica gel CC, eluted
with cyclohexane–CHCl3–MeOH (1:0:0, 1:1:0, 0:1:0, 0:100:1,
0:50:1, 0:10:1, 0:0:1 v/v) to give seven fractions A–G. Fraction C
was further purified by silica gel CC eluted with a gradient of
increasing CH2Cl2 in petroleum ether (60–90) to afford compounds
3 and 8, and fraction E with a gradient of increasing Me2CO
in petroleum ether (60–90) to afford compound 2. The n-BuOH extract
(193 g) was subjected to silica gel CC, eluted with a gradient
of increasing MeOH in CHCl3 to give fractions 1–6. Fraction 4 was
further purified by silica gel CC eluted with petroleum ether
(60–90)-Me2CO to afford compound 11. Fraction 5 was subjected
to silica gel CC to give three subfractions 1–3. Subfraction 1 was
further purified by CC on silica gel eluted with petroleum ether
(60–90)-EtOAc to afford compounds 1, 6 and 9. Subfractions 2
and 3were further purified by repeated silica gel CC eluted with
CH2Cl2–MeOH–H2O (10:1:0.1) to afford compounds 7, 10, 5 and
4. Compounds 1–11 were purified by Sephadex LH-20 CC eluted
with MeOH to afford 1 (500 mg), 2 (10.5 mg), 3 (8 mg), 4
(44 mg), 5 (6.2 mg), 6 (30.1 mg), 7 (13.2 mg), 8 (18 mg), 9
(30 mg), 10 (16.3 mg), 11 (100 mg), respectively.
4

ผลลัพธ์ (ไทย) 3:

สารสกัดจาก chcl3 ( 2.4 g ) ภายใต้ซิลิกาเจลซีซี , ตัวอย่าง
กับไซโคลเฮกเซน– chcl3 –เมทิลแอลกอฮอล์ ( 1:0:0 1:1:0 0:1:0 0:100:1 , , , , 0:50:1 0:10:1 0:0:1
, , v / v ) ให้เจ็ดเศษส่วนเศษส่วนเป็น–กรัม C
ยังบริสุทธิ์ โดยซิลิกาเจล มีความลาดชัน
ตัวอย่างซีซี เพิ่ม ch2cl2 ในปิโตรเลียมอีเทอร์ ( 60 - 90 ) เท่าใดสารประกอบ
3 และ 8 และเศษส่วน E มีความลาดชัน me2co
เพิ่มin petroleum ether (60–90) to afford compound 2. The n-BuOH extract
(193 g) was subjected to silica gel CC, eluted with a gradient
of increasing MeOH in CHCl3 to give fractions 1–6. Fraction 4 was
further purified by silica gel CC eluted with petroleum ether
(60–90)-Me2CO to afford compound 11. Fraction 5 was subjected
to silica gel CC to give three subfractions 1–3. Subfraction 1 was
further purified by CC on silica gel eluted with petroleum ether
(60–90)-EtOAc to afford compounds 1, 6 and 9. Subfractions 2
and 3were further purified by repeated silica gel CC eluted with
CH2Cl2–MeOH–H2O (10:1:0.1) to afford compounds 7, 10, 5 and
4. Compounds 1–11 were purified by Sephadex LH-20 CC eluted
with MeOH to afford 1 (500 mg), 2 (10.5 mg), 3 (8 mg), 4
(44 mg), 5 (6.2 mg), 6 (30.1 mg), 7 (13.2 mg), 8 (18 mg), 9
(30 mg), 10 (16.3 mg), 11 (100 mg), respectively.
4