2. Materials and Methods
2.1. The Concentrations of PGRs and Media Preparation. 1.0
× MS medium (1962) containing 0.25 mg L−1 GA3 , 1 mg L−1
NAA, 1 mg L−1 IAA, and 1 mg L−1 IBA with and without
0.25 mg L−1 GA3 complemented with 3% (w/v) sucrose and
0.8% (w/v) agar was used. The pH was calibrated to 5.6–
5.8 by 1 N HCl or 1 N NaOH after adding all medium
components except the agar. GA3 , IAA, and IBA are thermo-
labile; therefore, they were filter-sterilized by passing through
0.2