The results of the 23 design showed that the condition of
200 g L1 of TRS and temperature 30 C was the most
favorable, achieving 54.83 g L1 after a 48-hour-culture
time. The time was a decisive factor, once the ethanol production
increased to more than 60% from 24 to 48 h. By
comparison Bandaru et al. (2006) reported a maximum ethanol
concentration (55.3 g L1) at 32.4 C, pH of 4.93 after
17.24 h from sago starch using Z. mobilis MTCC 92. Davis
et al. (article in press) reported similar values (54 g L1) for
Z. mobilis ZM4 from hydrolysed waste starch stream.