eluted withMe2
CO and contain-ing a mixture of resin glycosides, was treated with activated charcoal to
eliminate pigmented residues. This fraction was resolved by HPLC on a
Symmetry C18column (Waters; 7μm, 19300 mm) with an isocratic
elution of methanol and a flow rate of 8 mL/min. Eluates across the
peaks withtRvalues of 19.8 min (peak I), 21.9 min (peak II), and 29.3
min (peak III) were collected by the technique of heart cutting and
independently reinjected ,