hich exist in a complex
symbiotic relationship [2, 3]. Recent investigations of
the microbiological composition of different types of kefir
granules have shown that lactic acid bacteria are present
as the largest portion (65–80%), whereas yeasts and other
microbial species are present in smaller proportion [4]. In
addition to these beneficial microorganisms, kefir contains
minerals, e.g., calcium, essential amino acids, and several
vitamins, e.g., folate [5]. Folate is relative to the nutritional
needs of humans, frequently among the most limiting of all
vitamins [6]. It is required for the synthesis of DNA and
RNA, which are primary events for cellular replication and
growth [7, 8]. Folate occurs in different forms in nature
which vary significantly in stability and bioavailability [6].
A sufficient intake of folate gives an increased protection
against megaloblastic anemia and child birth defects such
as neural tube defects [9–11]. However, a substantial part of
the population in European countries does not get enough
folate from foods [12]. There is therefore an interest both
from a general health perspective, as well as an opportunity
for industry, to develop new healthy products to increase
the folate content in foods