After determination of the optimal polymer/clay ratio for each effluent, larger volume vessels were prepared. For this purpose, 250-mL laboratory vessels were filled with 200 mL effluent and the corresponding amount (1 mL) of 10% nanocomposite suspension was added to a final ratio of 0.05% clay. The vessel was stirred with a magnetic stirrer at maximum speed for about 1 min, and then for an additional 2 min at low speed. Flocs were left to precipitate for 30 min. Turbidity was measured with a LaMotte 2020i turbidimeter, and electrical conductivity and pH were measured with an EXTECH EC500 pH/conductivity probe. TSSs were measured according to standard method 2540 [30], and electrokinetic charge was measured as described above. All parameters were measured before and after treatment.