The Yellow River Delta (YRD) is an important agricultural and ecological base that is being
threatened by crude oil contamination. The remediation of the YRD can be potentially assisted by
biosurfactants. Three native unknown biosurfactant-producing bacteria have been isolated (L1, L2, L3).In this paper, we measured the emulsification properties of the three biosurfactants in terms of emulsification activity (EA) and emulsification stability (ES) and compared them to two artificial surfactants, sodium dodecyl sulfate (SDS) and Triton X-100. We found the biosurfactants had comparable EA and superior ES to the two artificial surfactants.