Recently, we isolated and identified a novel Thraus- tochytrid microalga (termed Aurantiochytrium sp. KRS101) that produced high levels of lipid and DHA (over 45% total fatty acids) [8]. Upon fed-batch fermen-tation in a 5-L bioreactor, good biomass levels and lipid production values (50 and 22 g/L, respectively) were obtained; DHA productivity (3.3 g/L/d) comparable to that of S. limacinum SR21 [6]. To evaluate the comer- cial potential of such fermentation, large-scale growth of the microalgal strain was studied and the results are de- scribed in the present report. Also, it is worth extraction methods of fatty acid from fermentative sources in espe- cially industrial fields.