Abdominal writhing test
The antinociceptive effect of PIL extract (i.p) on the abdominal writhes of mice induced
by 0.6% acetic acid is summarized in Table 3. Intraperitoneal administration of PIL
extract had significantly inhibited the writhing effects in mice. Number of stretching
response observed in 15 minutes for control group (5% ethanol) was 51.7 ± 4.6 counts.
PIL at 10, 30, 100 and 300 mg/kg significantly reduced the writhings to 33.0 ± 3.9, 27.2 ±
7.7, 21.8 ± 3.7 and 20.3 ± 2.5 respectively.. Indomethacin (10 mg/kg), also given
intraperitoneally had also significantly reduced the writhings to 19.0 ± 2.4 counts, which
is almost comparable to PIL at 300 mg/kg (Table 3).