For sample preparation the following instrumental apparatus was used: vortex Biomixer, model QL-901 (Brazil); analytical balance precision AND, model SV‑200 (Japan); centrifuge Thermo Fisher Scientific, model IEC CENTRA CL2 (USA); centrifuge Centribio 12 × 15 mL (Brazil); automatic micropipette Brand with variable capacity (Germany) and thermostated water bath De Leo (Brazil).