Materials
Full-fat rice bran (Hitomebore) was purchased from a
local farmer in Sendai, Japan. Rice bran was defatted by
extracting twice with 3 vol. of hexane. The defatted rice
bran (DRB) was stored at 5 C until use. Glycerol was
purchased from Sigma Chemical (St Louis, MO, USA).
Celite No 545 was purchased from Wako Pure Chemicals
(Osaka, Japan). Commercial SP was obtained from
Fuji Oil Co. (Osaka, Japan). All other reagents were of
analytical grade.