The 16S rDNA gene of bacteria
was amplified by the first polymerase chain reaction (PCR)
with universal primer 27f (GAGTTTGATCCTTGGCTCAG) and
1525r (AAGGAGGTGWTCCARCC). 16S rDNA gene for archaea was
amplified using Arch21f primers (TTCCGGGTTGATCCYGCCGGA)
and Arch958r (YCCGGCGTTGAMTCCAATT).