Temperature management during storage is the single most important factor in minimizing the deterioration of fruit quality and extending the shelf life of strawberry. However, in Bangladesh most of the growers and retailers store their product at ambient condition due to lack of cooling facility. Storing at ambient temperature makes postharvest management of strawberries very difficult (Asrey et al., 2004), as higher storage temperature results in higher respiration rates, which is associated with reduction of fruit quality (Ayala-Zavala et al., 2004). While, cultivar quality is defined by different parameters, which unitedly gives an integral picture of selected fruit. However, this research was undertaken to examine the effect of maturity stage and cultivar on selected qualitative parameters in strawberry fruits at ambient temperature and determine the proper harvesting time so that optimum state of their combination can be attained under sub tropical climate.