2.7. Extraction of lentil seeds
For extraction of phenolics, lentil seeds were homogenized in
80% methanol in ratio 1:5 (fresh weight:methanol), using Fast
Prep-24 System (M.P. Biomedicals, Irvine, CA, USA). Sample was
homogenized in two intervals of 40 s at speed of 6.5 m s1.
Afterwards, homogenates were centrifuged at 10,000 g for
20 min and supernatants were used for further analyses. Four
extracts were prepared for each sample analyzed.