The present findings demonstrate that, although FID and various
MS techniques exhibit distinct behavior where response to
FAME for quantification is concerned, the use of calibration
with internal standardization allows the application of MS to
quantitative FAME analysis with adequate precision, low LOD,
and accurate quantitative results. The hallmarks of FID performance
in FAME quantification include a broad range of linear
response, excellent precision, and subpicomole LOD. Of
course, the main limitation of this detector is that the response
is nonspecific and provides no information on the identity of
the analyte.