Prediction of miRNAs targeting Cf-9
MicroRNAs targeting the Cf-9 gene was predicted using the
SoMART database (http://somart.ist.berkeley.edu) as described
[18]. Briefly, the Cf-9 mRNA sequence was input and potential
miRNAs targeting this gene were predicted using Slicer Detector
program and SLY1 as tomato sequence database. The targets of the
predicted miRNAs were confirmed using dRNA Mapper program
with degradome database. The pre-miRNA sequences of the predicted
miRNAs were obtained using PreMIR Detector program.