Lipid content was measured by the acid hydrolysis method. The MLE (1 g) were mixed with hydrochloric acid (20 ml) in
a conical flask, then heated in a water bath at 70–80 C for about 50 min. After heating, the mixture was cooled to
room temperature, and then 10 mL of ethanol and 20 ml of ethyl ether were added to a separatory funnel. The separatory funnel was vigorously shaken to ensure complete mixing of the two liquid phases. Then, the two liquid
phases were allowed to separate for at least 2 h until the layers were clearly separated. The lower solvent was collected in a new beaker, and the upper solvent (ethyl ether) was collected in a conical flask. Ethyl ether (20 ml) was
added to the lower solvent and poured into a separatory funnel, which was shaken vigorously to ensure complete