The double packed sample pouches were treated in a batch mode high-pressure vessel (model: S-IL-100-250-09-W; maker: Stansted Fluid Power, UK). The pressurization was accomplished by compressing 30% mono-propylene glycol which served as the transmitting medium. The pressurizing rate was fixed at 300 MPa·min− 1 and a rapid decompression was achieved (< 10 s). The maximum target pressure (600 MPa) was reached within 133 s after the cycle starts; whereas for 200 MPa, the corresponding figure was 45 s. The target temperature near the sample was maintained by the circulating fluid in the jacket surrounding the vessel. The hysteresis set for pressure and temperature was 5 MPa and 1 °C, respectively. After the treatment, a refrigerated condition (2–4 °C) was ensured for the samples prior to biochemical analysis.