The tocopherol content was determined by standard method
ISO 9936:2006. The chromatographic system consisted of a Liquid
Chromatograph HP 1090 (Hewlett–Packard) and fluorescence
detector HP 1100 (kex = 300 nm, kem = 330 nm). The
analytical column was InterSil Si (5 lm, 150 4.6 mm) (Chrompack).
The mobile phase was a 0.3% solution of ethanol in hexane,
at a flow rate 1.0 ml/min. The samples were prepared by classical
treatment, which included saponification with KOH and ethanol.
The extraction of unsaponificable matters was performed with
diethyl ether.
b-Glucan content of samples was determined enzymatically, in
accordance with the method of McCleary and Glennie-Holmes
(1985) modified by McCleary and Nurthen (1986).
All determinations were performed in triplicate.