Physical allurement leads to the formation of physical gels by weaker linkages through either van der Waals forces, hydrophobic, electrostatic interactions,or hydrogen bonding. These systems are sensitive depending on polymer composition. temperature. ionic strength of the medium, concentrations of the polymer and of the cross-linking agent (Sultana et al., 2013). Covalent conjugation results in the production of chemical gels comprising of permanent chemical linkages or covalent bonds throughout the gel networks. In fact. the properties of such gels depend on the chemical linkages and functional groups present in the gel networks (Sultana et al.. 2013). The self-assembly procedure or the self- organization of components into structurally well-defined aggregates has unique advantages including being cost-effective. simple and flexible and more importantly the process occurs toward the system's thermodynamic minima. resulting in stable and robust structures (Goncalves, 2010). For instance,by using the self-assembly sys- tem unique monodispersed nanogel with a hydrophobic core and hydrophilic shell could be produced (Akiyoshi. 2002) Nanogels could also be developed by using natural polysaccharide e g. chitosan (CS). pollulan. dextran and etc... through physical interaction. Cs. a(1-4) 2 amino-2-deoxy B-D-glucanj, a deacety lated form of chitin, is an abundant positively-charged hydrophilic polysaccharide possessing weak basic groups (Kato et al 2007). Cs