the bacterium was pre-cultured in nutrient broth overnight in a rotary shaker at 37°C and the cell density was standardized spectrophotometrically (A610 nm) equaled McFarland standard 0.5 in nutrient broth. The other procedures were same as above.
the bacterium was pre-cultured in nutrientbroth overnight in a rotary shaker at 37°C and the cell density was standardized spectrophotometrically (A610nm) equaled McFarland standard 0.5 in nutrient broth. The other procedures were same as above.