L. sakei subspecies sakei JCM 1157 and L. mesenteroides subspecies
mesenteroides NBRC 100496 were adhered by drying onto
stainless steel dishes. To produce cultures, the bacterial cells were
inoculated into 20 mL Gifu Anaerobic Medium (GAM) broth (Nissui
Pharmaceutical, Tokyo) and incubated at 30 C for 72 h. Under these
conditions, the cultures reached stationary phase.