Digestion of the amplicons NS1-NS24 and ITS1-ITS4 with different restriction enzymes resulted in banding patterns summarized in Table 2. Nine strains from saltpans and seawater proved tobe close to reference strains of hortaea weneckii. Only with HinfI some variation was found. In 9 out of 20 strains of the entire panel, including 6 strains in slovenia and one from croatia, a pattern clearly different from that of the ex-neotype strain, CBS 107.67, was found (plate 1e).