A standard curve of glucose was prepared from different
standard glucose solution in different test tubes containing
0.0, 10, 20, 40, 60, 80 and 100 lg of glucose respectively and
the volume was made up to 1 ml with distilled water. Then
4 ml of the anthrone reagent was added to each test tube
and mixed well. All these solutions were treated similarly as
described above. Absorbance was measured at 620 nm using
the blank containing 1 ml of water and 4 ml of the anthrone
reagent. The amount of total sugar present in the extract
was calculated from the standard curve of glucose. Finally
the percentage of total sugar was determined.