The plant samples (20 g) were powdered using an electric blender and macerated three times with 95% ethanol (200 ml) for 72 h at room temperature. Subsequently, each extract was filtered through Whatman No. 1 filter paper and evaporated to dryness at 50°C. Ten mg of each extract was dissolved in ethanol to a total volume of 1 ml and was then filtered through 0.22 mm Millipore filter for HPLC analysis.