. 10 L of medium containing 200 g L−1
glucose, 5 g L−1 yeast extract, 0.5 g L−1 KH2PO4, 0.02 g L−1 MgSO4·7H2O, 0.02 g L−1
Fe(NH4)2(SO4)2·6H2O, 0.001 g L−1 biotin and 0.002 g L−1 calcium panthotenate were
inoculated with 1 L preculture grown on the same medium. R