Chitosan is known as a cheap, eco-friendly and efficient biosorbent
for pollutant mixtures. In the treatment of textile effluents
treatment, it has been widely applied for dye removal and/or
decolorization [1]. The amino (–NH2) and hydroxyl (–OH) groups
of chitosan serve as the active sites for biosorption. The chemical
modification of this water-insoluble material can be done easily
because of the presence of these groups [2–4]. ] The elemental structure of chitosan
has no difference after this acidic modification. The chitosan beads
(CBs) have a higher adsorption capacity than the raw chitosan
flakes.
Laccase has been of interest in recent years for synthesizing various
dyes and decolorizing industrial textile effluents [5]. It catalyzes
the oxidation of substrates coupled with the reduction of
molecular oxygen from water. Laccase was effectively and popularly
used for dye removal [6,7]. Under alkaline conditions, the
hydroxide ion causes enzyme inhibition [8].