SThe column speed was adjusted using approximately 2 psi of argon, fed through the top of the column. The eluted oil was kept chilled unit the stripping procedure was complete, and then the oil was dried under rotary evaporation using an R-215 rotary evaporator(Buchi, Newcastle, Del., U.AS.A.) WITH A V-855 vacuum controller.
The oil was further stored under high vacuum for 4 h to remove trace amounts of hexane.
The sttipped oil was checked by HPLC to verify that tocopherols had beed adequately removed during oil stripping (Winkler-Moser and others 2014)