documented that highly
degraded DNA could not be detected if P300 bp amplicons are
used. Furthermore, earlier multiplex PCR assays included only pig
and horse meats among the haram (not allowed) meat species
(Kitpipit et al., 2014; Matsunaga et al., 1999), which have limited
scope in halal authentication. Here we developed a multiplex
PCR system with self-designed species-specific primers targeting
short-length amplicons (108–172 bp) of target species to detect
five most potential haram meat species having health risk and
wider scope in food traceability studies.