The FAME content dropped to 45% and 3% when 6 or 9 molar equivalents of methanol were added, respectively. Interestingly, when the immobilized lipase from T. Lanuginosus was used, the conversion topped at 98% in the reaction mixture of oil/methanol (1:6). This is essentially full conversion and the only reaction in which 6 molar equivalents of methanol results in better FAME yield compared to 3 equivalents. In the model study of methanol tolerance, CALB came out as superior to TLL (Fig. 3).