the generation time of Lactococcus spp. becomes longer. However, Bacillus sp., because of its aciduric nature, can then start multiplication, metabolize proteins, and increase the pH (say 5.8). In the high pH, the Pseudomonas spp. present initially can then grow by metabolizing NPN and proteinaceous compounds and increase the pH further by producing basic metabolites (amines, NH3). This way, the predominant spoilage microorganisms and the metabolites associated with spoilage (e.g., nature of spoilage) of a food can change if a food is stored for a fairly long time.3