Conclusion
The optimization study to increase the product by controlling
the variables was successfully performed using response surface
methodology from the data obtained from Plackett-Burman design
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trial plus 3 central points. From the response surface graphs the
optimum conditions were selected to be jaggery (40% w/v), inoculums
volume (8% v/v) and incubation temperature (30°C). The results states
that the alcohol production is inhibited at higher jaggery concentration
(60% w/v) and at higher incubation temperature (35°C). Varying the
volume of inoculums between the range 8 to 12% v/v did not show any
significant effect and hence inoculums volume added to media can be
fixed at any value within this range. The highest amount of alcohol (i.e.,
95.84 g/l) was obtained after process optimization. This value was close
to the value obtained from model equation 98.70 g/l. Thus, finally we
conclude that the strain S. cerevisiae Jm.20 could be efficiently used as
an inoculums for fermentation process at optimal conditions.