Bias can also be caused in a cyclone by the pulsation of the personal sampling pump. Bartley, et al. [12] showed that cyclone samples with pulsating flow can have negative bias as large as 0.22 relative to samples with steady flow. The magnitude of the bias depends on the amplitude of the pulsation at the cyclone aperture and the dust size distribution. For pumps with instantaneous flow rates within 20% of the mean, the pulsation bias magnitude is less than 0.02 for most dust size distributions encountered in the workplace.