The control as well as puris containing 0.5% w/w guar gum were
analyzed for different nutritional quality parameters such as ash
content, protein content according to AACC methods (2000). IVSD
(in-vitro starch digestibility) was determined by subjecting the
samples to digestion using acetate buffer (pH-4.6) by amyloglucoxidase
at 60 C for 30 min, boiled for inactivation, cooled and
centrifuged (Goñi, García-Alonso, & Saura-Calixto, 1997). The
supernatant was checked for available glucose, estimating by
glucose oxidase peroxidase assay and measuring the absorbance at
505 nm which is directly proportional to the glucose concentration.
IVPD (in-vitro protein digestibility) was estimated using pepsinpancreatin
protein digest index method (Akeson & Stahmann,
1964). The nitrogen content of the sample and the undigested
residue were determined by the Microkjeldahl method (AACC,
2000). The digestible protein of the sample was calculated by
subtracting residual protein from total protein of the sample.
The control as well as puris containing 0.5% w/w guar gum wereanalyzed for different nutritional quality parameters such as ashcontent, protein content according to AACC methods (2000). IVSD(in-vitro starch digestibility) was determined by subjecting thesamples to digestion using acetate buffer (pH-4.6) by amyloglucoxidaseat 60 C for 30 min, boiled for inactivation, cooled andcentrifuged (Goñi, García-Alonso, & Saura-Calixto, 1997). Thesupernatant was checked for available glucose, estimating byglucose oxidase peroxidase assay and measuring the absorbance at505 nm which is directly proportional to the glucose concentration.IVPD (in-vitro protein digestibility) was estimated using pepsinpancreatinprotein digest index method (Akeson & Stahmann,1964). The nitrogen content of the sample and the undigestedresidue were determined by the Microkjeldahl method (AACC,2000). The digestible protein of the sample was calculated bysubtracting residual protein from total protein of the sample.
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