The antioxidant activity of the shiitake extract was determined using scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals test following the method of Fu, Shieh, and Ho (2002) with some modifications. Briefly, 2.9 mL of DPPH solution (0.5 mM) was added to 0.1 mL of ethanol solution containing different concentrations of shiitakes extracts (1, 2, 3 and 5 mg/mL). The mixture was shaken and allowed to stand at room temperature in dark for 30 min. The absorbance of the solution at 517 nm was measured using a