The majority of studies that address the regulation of α-TTP levels have focused on humans and rodents, while very little is known regarding the sheep α-TTP. In our previous research we have proved the existence of the α-TTP gene in sheep and for the first time, cloned the full-length cDNA of the ovine α-TTP gene using Aohan fine-wool
sheep and Tan sheep, respectively (Jia et al., 2012; Liu et al., 2012). In the present study, we report ourfindings regarding the regulation of sheep α-TTP by dietary vitamin E using quantitative real-time PCR (qRT-PCR) and Western-blot analysis. Monoclonal antibodies (McAb) against ovine α-TTP were also produced and used in immunohistochemical studies to analyze the sheep α-TTP expression levels under different vitamin E supplementations and to more precisely clarify its biological function