Methods: Six groups (n=18) of restored dentin samples were prepared using amalgam, a
microhybrid, a nanohybrid and a silorane composite. The composites were adhesively bonded
with systems with or without an antibacterial monomer (Clearfil-SE-Protect, Clearfil-SE-bond,
respectively), except for the silorane group (Silorane- System-Adhesive). Non-restored dentin
samples were used as control (primary caries). Samples were inserted into slots, in lower
prosthesis especially made for the experiment. Subjects were instructed to dip the lower prosthesis
in a sucrose solution 4 times per day. At baseline and 8 weeks, samples were radiographed extraorally
and the integrated mineral loss was calculated. Data were statistically analyzed using
multiple linear regression with a multilevel model (p=0.05).