Regardless of varieties of pigmented ricebran, the contents of the total phenolic and the total flavonoid appeared highest in 40% acetone solvent. In particular, the contents of the total phenolic and the total flavonoid showed a strong correlation with the DPPH radical scavenging ability. This could most likely be attributed to different kinds of antioxidant compounds particularly anthocyanin and proanthocyanin in black and red rice bran (Kong and others 2008; Yao and others 2010). In addition, the antioxidant activity of these components may vary depending on extraction solvent used. The reason for this phenomenon is due to the solvent has a major effect on the composition of the antioxidative compounds (Alothman and others 2009; Lai and others 2009). Finocchiaro and others (2010) found that the DPPH radical scavenging ability
of barley was in decreasing order for a 70% acetone extract, 70% ethanol extract, and a 70% methanol extract, indicating that there were differences in DPPH radical scavenging ability of barley depending on the extraction solvent. Therefore, solvent choice is very important because both contents of antioxidants and antioxidant activity are influenced by solubility in the extraction solvents. Considering the DPPH radical scavenging ability as well as the contents of total phenolic and flavonoid, the best result was obtained from 40% acetone. Therefore, we selected 40% acetone as an extraction solvent for antioxidant study of pigmented rice bran.
Regardless of varieties of pigmented ricebran, the contents of the total phenolic and the total flavonoid appeared highest in 40% acetone solvent. In particular, the contents of the total phenolic and the total flavonoid showed a strong correlation with the DPPH radical scavenging ability. This could most likely be attributed to different kinds of antioxidant compounds particularly anthocyanin and proanthocyanin in black and red rice bran (Kong and others 2008; Yao and others 2010). In addition, the antioxidant activity of these components may vary depending on extraction solvent used. The reason for this phenomenon is due to the solvent has a major effect on the composition of the antioxidative compounds (Alothman and others 2009; Lai and others 2009). Finocchiaro and others (2010) found that the DPPH radical scavenging abilityof barley was in decreasing order for a 70% acetone extract, 70% ethanol extract, and a 70% methanol extract, indicating that there were differences in DPPH radical scavenging ability of barley depending on the extraction solvent. Therefore, solvent choice is very important because both contents of antioxidants and antioxidant activity are influenced by solubility in the extraction solvents. Considering the DPPH radical scavenging ability as well as the contents of total phenolic and flavonoid, the best result was obtained from 40% acetone. Therefore, we selected 40% acetone as an extraction solvent for antioxidant study of pigmented rice bran.
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