All analytical experiments were carried out in triplicate. In order
to evaluate an influence of three different factors (fermentation
method, the type of microorganisms, and lupin variety) and their interaction on free essential and non-essential amino acids, and
biogenic amines content in lupin seeds, data were subjected to
analysis of the three-way analysis of variance (ANOVA) and the
Tukey HSD test as post-hoc test (statistical program R 3.2.1, R Core
Team 2015). The results were refered to statistically significant at
P 0.05.