Potato pulp by-product rich in galactan-rich rhamnogalacturonan I (RG I) was investigated as a new
source of oligosaccharides with potential prebiotic properties. The efficiency of selected monocomponent
enzymes and multi-enzymatic preparations to generate oligosaccharides/oligomers from potato RG I was
evaluated. These overall results of yield were dependent on the activity profile of the multi-enzymatic
preparations. Highest oligo-RG I yield of 93.9% was achieved using multi-enzymatic preparation (Depol
670L) with higher hydrolytic activity toward side chains of RG I as compared to its backbone. Main
oligo-RG I products were oligosaccharides with DP of 2–12 (79.8–100%), while the oligomers with DP
of 13–70 comprised smaller proportion (0.0–20.2%). Galactose (58.9–91.2%, w/w) was the main monosaccharide
of oligo-RG I, while arabinose represented 0.0–12.1%. An understanding of the relationship
between the activity profile of multi-enzymatic preparations and the yield/DP of oligo-RG I was achieved.
This is expected to provide the capability to generate galacto- and galacto(arabino) oligosaccharides and
their corresponding oligomers from an abundant by-product.