2.8. Statistical analysis
A one-way Anova with Duncan post-hoc comparison of means and an independent-samples t test were used. Significance was determined at P < 0.05 using the SPSS statistical software (v12.0, Chicago, IL, USA). HPLC-ESI-MS analysis was conducted with three replicate in two separate experiments. The MTT assay, cell cycle analysis, caspase apoptotic assay and TUNEL assay were determined in three separate experiments with three replicate.