Before the start of the study, participants underwent a complete medical screening (medical history, physical examination, and resting electrocardiogram) to ensure that there were no contraindications to study participation. An incremental cycle ergometer test up to exhaustion was also performed for the determination of peak oxygen consumptio (V˙O2 peak) and peak work rate (41.2 ± 4.7 mL·kg−1·min−1; 226 ± 49 W).