All the aspects influencing fiber adsorption (time, temperature, pH, salt addition) and desorption
(desorption and injection time, desorption solvent mixture composition) of the analytes have been investigated.
An isocratic separation was performed using an acetonitrile-phosphate buffer (pH 2.8; 2 mM)
mixture (70:30, v/v) as the mobile phase. The estimated LOD and LOQ values (g/mL) were in the range
0.002–0.028 and 0.007–0.095. The within-day and day-to-day precision values (RSD%) were between
4.7–6.1 and 6.6–9.4, respectively.