A Kromasil C18 column provided
chromatographic separation of analytes followed by detection with mass spectrometry. The method involves
simple isocratic chromatography conditions and mass spectrometric detection in the negative ionization mode
using a Waters Xevo TQS MS/MS system. The proposed method has been validated with a linear range of 1.00 –
1000ng/mL and 5 – 10000ng/mL for AA and CA respectively