As shown in Table 1, there are significant differences in the
callus induction data arising from the variations of the plant
growth media and explants used with respect to callus initiation and growth. It is seen that all explants (leaf, stem and corm) of the five crocus species cultured on the four nutrient media (MS, GB5, LS and CHE’) without any plant growth regulator did not show any formation of callus, but the explants remained viable for up to 2 months.