Bovine 1-adrenergic receptor (AR) cDNA was cloned using degenerative primers. Bovine 횩1-AR coded for 467 amino acids and the comparison of the deduced amino acid sequence with that of sheep showed 93.4% identity. Northern blot analysis indicated that transcript size for the bovine 횩1-AR was 3.6 kb in the adipose tissue. The expression level of three -ARs (1, 2, and 3) in bovine abdominal, subcutaneous, and perirenal adipose tissues were examined using reverse transcription-polymerase chain reaction (RT-PCR), and the levels of 횩1- and 3-AR mRNA were found to be lower in the subcutaneous adipose tissue than in the abdominal and perirenal adipose tissues. These results suggest that the expression of 횩-ARs mRNA are differentially regulated among the adipose tissues.