2.6. Characterization of SBE
2.6.1. HPLC analysis of phenolic acid and flavonoids
The analyses were performed by using a Shimadzu liquid
chromatograph (Kyoto, Japan) equipped with an SIL-20 auto sampler,
an SPD-M10A VP PDA photodiode array detector and Class VP 7.3
chromatography software. A C18 reversed phase column (Phenomenex
Kinetex®2.6 μm, 100A, 100 × 4.60mmi.d.)was used as the solid phase