As fat has
low solubility in water, half-sized quantities (compared to that described in Section
2.7) were used in order to achieve the proportion of fat in the BOD7 of combined
synthetic wastewater of approximately 15–20%. Additionally combined wastewater
dosed with milk or CMC was used, by adding 1 ml milk with fat content of 2.5% or
1 g CMC, respectively, into 1000 ml of OECD synthetic wastewater.