Approximately 300 μg of purified DNA from submucosal tissues were
processed through the LOOXSTER® Enrichment Kit according to the
manufacturer's instructions. Briefly, the volume of the DNA sample was
adjusted and an equal volume of binding buffer added, followed by the
addition of LOOXSTER® magnetic particles. Using a magnetic separator,
bound particles were washed several times on a thermomixer and the
particle-free supernatant discarded. Particles were disassociated,
removed from the mixture using a magnetic separator, and the supernatant added to the LOOXSTER® desalting spin column. After several
washes, the DNA was eluted from the column in 40 μl total volume
Approximately 300 μg of purified DNA from submucosal tissues wereprocessed through the LOOXSTER® Enrichment Kit according to themanufacturer's instructions. Briefly, the volume of the DNA sample wasadjusted and an equal volume of binding buffer added, followed by theaddition of LOOXSTER® magnetic particles. Using a magnetic separator,bound particles were washed several times on a thermomixer and theparticle-free supernatant discarded. Particles were disassociated,removed from the mixture using a magnetic separator, and the supernatant added to the LOOXSTER® desalting spin column. After severalwashes, the DNA was eluted from the column in 40 μl total volume
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